Our Tests

K9-TMB/MSI™

What is K9-TISSUE TMB/MSI™? 

K9-TISSUE TMB/MSI™ is an advanced genomic test designed to provide detailed molecular  profiling of canine tumours. By analysing 499 cancer-related genes, the test identifies important  genetic alterations within a tumour, helping veterinarians make more informed treatment  decisions and support a personalised approach to cancer care.

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What does the test analyse? 

K9-TISSUE TMB/MSI™ detects clinically relevant genetic alterations across 499 cancer-related  genes, providing valuable information about tumour biology, prognosis and potential therapeutic  targets. 

The test also evaluates two key immunotherapy biomarkers alongside genomic profiling: – Tumour mutational burden (TMB) measures the number of genetic mutations within a tumour.  Certain tumours with a high mutational burden may be more likely to respond to  immunotherapy. 

– Microsatellite instability (MSI) assesses defects in the tumour’s DNA repair system. Identifying  MSI can provide additional information about tumour behaviour and may influence treatment  options in selected cases. 

K9-TISSUE TMB/MSI™ is the only panel currently available on the market that combines both TMB  and MSI characterisation. 

Sample requirements 

K9-TISSUE TMB/MSI™ can be performed using: 

  • Formalin-fixed, paraffin-embedded (FFPE) tumour tissue 

 

We require 5 x 5 µm shavings from tumour blocks containing at least 30% tumour tissue. 

Please note that the process used to remove minerals from bone tissue (decalcification) can  damage tumour DNA, which may make genetic testing unsuccessful. If you are planning  genetic testing in the future, please ask your pathologist to save a sample of soft tumour  tissue before decalcification is performed. This is especially important for cancers such as  osteosarcoma, oral melanoma, and oral squamous cell carcinoma. 

  • Fine-needle aspiration (FNA) samples. 

 

We require at least three cellular slides containing at least 30% tumour cells. 

Your veterinarian will determine which sample type is most appropriate based on your dog’s  diagnosis and the available tissue.

We provide all necessary packaging, shipping documentation and instructions to ensure samples  are securely transported to the laboratory. Results are typically available within three weeks of  sample collection by the veterinary team. 

Why is genomic profiling important? 

Every cancer is genetically unique. Genomic profiling helps veterinarians understand the  individual characteristics of a tumour rather than relying solely on its location or type. This  information can support more personalised treatment planning, identify potential targeted  therapies or immunotherapy options, and provide additional insight into prognosis. 

Supporting personalised cancer care 

Precision medicine is becoming an increasingly important part of veterinary oncology. K9-TISSUE  TMB/MSI™ provides comprehensive genetic information that helps veterinarians better  understand each patient’s cancer and tailor treatment decisions to the tumour’s unique  molecular profile. 

By combining extensive genomic analysis with the assessment of TMB and MSI, K9-TISSUE  TMB/MSI™ represents an important step towards more personalised cancer management for  dogs. 

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K9-TMB/MSI™ 499-Gene Panel

Genes included in the K9-TMB/MSI™ genomic profiling assay.

ABCB1BTKCUX1ETV4GATA4JAK1MEF2BPAX5PTPRJSLC45A3
ABCC2BUB1BCXCR4ETV5GATA6JAK1MEN1PBRM1PTPROSLCO1B1
ABL1CACNA1DCYLDEWSR1GLI1JAK2METPCQKISLIT2
ACTBCALRCYP19A1EXOC2GLUD2JAK3METPCGF2RAB3GAP2SOCS1
ACVR1BCARD11CYP2A6EXT2GNA11JARID2MGMTPDGFBRAC1SOS1
ADGRA2CBFBCYP2B6EZH2GNA13JUNMITFPDGFRARACQL4SOX10
AIPCBLCYP2C19FAM133BGNAQKAT6AMITFPDGFRBRAD21SOX2
AKT1CCN6CYP2C9FAM90A1GNASKDM2BMLH1PDK1RAD50SOX9
AKT2CCND1CYP2D6FANCAGNB1KDM5AMLLT10PDKF6RAD51SRC
AKT3CCND2CYP3A4FANCBGRIN2AKDM5CMPLPHOX2BRAF1STAT3
ALDH2CCND3CYP3A5FANCCGRM3KDM6AMRE11PIK3C2BRANBP2STAT4
ALKCCNE1DAXXFANCD2GSK3BKDRMSH2PIK3C2GRARASTAT5A
AMER1CCT6EDDR1FANCEGSTM1KDRMSH3PIK3C3RARBSTK11
ANKRD52CDADDR2FANCFGSTP1KEAP1MSH6PIK3CARARGSTK11
AP3B1CDC73DDX3XFANCGGSTT1KELMST1RPIK3CARASA1STX11
APCCDH1DHFRFANCLH3-3AKITMTHFRPIK3CBRASGEF1ASYK
ARCDK10DICER1FASHBA1KITMTORPIK3CDRB1TAF1
ARAFCDK12DLG2FAT1HBA2KLHL6MUC16PIK3CGRBM10TANGO2
ARFRP1CDK4DMDFAT4HBBKMT2AMUTYHPIK3R1RELNTANK
ARID1ACDK6DNM2FBXO11HDAC1KMT2BMYCPIK3R1RETTBC1D26
ARID2CDK8DNMT3AFBXO32HDAC2KMT2CMYCLPIK3R2RHOATEK
ARID58CDKN1ADOT1LFBXW7HDAC4KMT2DMYCNPLCG1RICTORTERT2
ARPC1ACDKN1BDPYDFBXW7HDAC7KMT2DMYD88PLCG2RNF43TET2
ATMCDKN1CDSPFCGR2BHGFKRASNBNPLK1ROS1TGFBR2
ATRCDKN2BDUSP2FGF10HNF1AKRASNCOR1PMS1RPS6KB1TMPRSS2
ATRXCDKN2CEBF1FGF14HNF1BLAMA2NCSTNPOLD1RPTORTP53
AURKACDKNA2AECT2LFGF19HRASLCKNEK2POLERRM1TRAF3
AURKBCEBPAEEDFGF23HSD3B1LEF1NELL2POT1RUNX1TRAF5
AXIN1CEP57EGFRFGF3HSP90AA1LMO1NF1PP2R1ARUNX1T1TSC1
AXLCHD2EGR1FGF6ID3LRP1BNF1PPM1LRUNX2TSC2
B2MCHD4EP300FGFR1IDH1LRP1BNF2PRDM1RXRATSHR
BAP1CHD7EPCAMFGFR2IDH2LRP2NFE2L2PREX2RXRBTTF1
BARD1CHEK1EPHA3FGFR3IGF1RLTKNFKBIAPRF1RXRGTTN
BCL2CHEK2EPHA5FGFR4IGF2LYNNKX2-1PRKAR1ASATB1TUBB3
BCL2L1CHIC2EPHA7FHIKBKELYSTNOTCH1PRKC1SBDSTYK2
BCL2L2CICEPHB1FIP1L1IKZF1LZTR1NOTCH2PRKDCSCRN1USH2A
BCL6CKS1BERBB1FLCNIKZF2MAGI2NPM1PRKNSDHAVEFGA
BCORCLHC1ERBB2FLT1IKZF3MAP2K1NQO1PRMT3SDHBVHL
BCORL1cOR8S14ERBB3FLT3IL2RAMAP2K2NRASPSMA1SDHCWEE1
BHLHA9CREBBPERBB4FLT4IL2RBMAP2K4NRG1PSMB1SDHDWRN
BLMCRKLERCC1FOXL2IL2RGMAP3K1NSD1PSMB2SEPTIN9WT1
BMPR1ACRLF2ERCC2FOXP1INHBAMAP3K14NT5C2PSMB5SERP2XPO1
BRAFCSF1RERCC3FRS2INPP4BMAPK1NTRK1PSMD1SETBP1XRCC1
BRCA1CSF3RERCC4FSTL5INPP5DMCL1NTRK2PTCH1SETD2YAP1
BRCA2CSMD3ERCC5FUBP1IRAK4MDM2NTRK3PTENSGK1YES1
BRD4CTCFERGGABRA6IRF1MDM4NUP93PTGFRSHHZAP70
BRIC3CTNNA1ERRFI1GADD45BIRF4MECOMORC1PTPN11SLC22A1ZBED4
BRIP1CTNNB1ESR1GATA1IRF8MECOMPAG1PTPN2SLC22A2ZBTB2
BTG1CTNNB1ESR2GATA2IRS2MED12PAK3PTPN5SLC31A1ZMYM3
BTG2CUL3ETV1GATA3ITCHMED13PALB2PTPN6SLC34A2–